rnascope 2.5 hd duplex detection kit (chromogenic)  (Advanced Cell Diagnostics Inc)

Journal: Frontiers in Immunology

Article Title: Mucosal and Systemic Immune Responses to Salmon Gill Poxvirus Infection in Atlantic Salmon Are Modulated Upon Hydrocortisone Injection

doi: 10.3389/fimmu.2021.689302

Figure Lengend Snippet: Singleplex in situ hybridization (RNAscope) showing detection of SGPV B22R1 and D13L transcripts in gills from Atlantic salmon at 1 and 3 days after exposure to SGPV (dpe). Whole gill serial sections from 1 dpe (n=3) were scanned for staining of B22R1 and D13L RNA, as marked (A, B) , revealing significant differences in detection of SGPV-positive cells (C) . Images of the SGPV-exposed sham injected (E.S) group from 1 dpe stained with B22R1 probe (D) and D13L probe (E) . Serial section from the E.S group from 3 dpe stained with B22R1 probe (F) and D13L probe (G) .

Article Snippet: RNAscope ® 2.5 HD Duplex Detection Chromogenic kit (Advanced Cell Diagnostics) was used for simultaneous detection of SGPV-D13L and salmon GzmA in Atlantic salmon gills from all groups at 7 dpe (n (E.S) = 3, n (E.H) = 3, n (C.S) = 1, n (C.H) = 1) along with one section from the E.S group and the E.H group at 14 dpe.

Techniques: In Situ Hybridization, RNAscope, Staining, Injection

Journal: Frontiers in Immunology

Article Title: Mucosal and Systemic Immune Responses to Salmon Gill Poxvirus Infection in Atlantic Salmon Are Modulated Upon Hydrocortisone Injection

doi: 10.3389/fimmu.2021.689302

Figure Lengend Snippet: Duplex in situ hybridization (RNAscope) demonstrating the distribution of SGPV (D13L, blue staining) and salmon GzmA (red staining) in gills from Atlantic salmon. (A) E.S group at 7 dpe. (B) E.H group at 7 dpe. (C) C.S group at 7 dpe. (D) C.H group at 7 dpe. (E) E.S group from 14 dpe. (F) E.H group at 14 dpe. Arrow show interaction between GzmA expressing cell and SGPV-infected cell.

Article Snippet: RNAscope ® 2.5 HD Duplex Detection Chromogenic kit (Advanced Cell Diagnostics) was used for simultaneous detection of SGPV-D13L and salmon GzmA in Atlantic salmon gills from all groups at 7 dpe (n (E.S) = 3, n (E.H) = 3, n (C.S) = 1, n (C.H) = 1) along with one section from the E.S group and the E.H group at 14 dpe.

Techniques: In Situ Hybridization, RNAscope, Staining, Expressing, Infection

Journal: Frontiers in Immunology

Article Title: Mucosal and Systemic Immune Responses to Salmon Gill Poxvirus Infection in Atlantic Salmon Are Modulated Upon Hydrocortisone Injection

doi: 10.3389/fimmu.2021.689302

Figure Lengend Snippet: In situ hybridization (RNAscope) targeting GzmA (stained in red) in spleen from Atlantic salmon at 14 dpe. (A) SGPV-exposed hydrocortisone-injected (E.H) group. (B) SGPV-exposed sham-injected (E.S) group.

Article Snippet: RNAscope ® 2.5 HD Duplex Detection Chromogenic kit (Advanced Cell Diagnostics) was used for simultaneous detection of SGPV-D13L and salmon GzmA in Atlantic salmon gills from all groups at 7 dpe (n (E.S) = 3, n (E.H) = 3, n (C.S) = 1, n (C.H) = 1) along with one section from the E.S group and the E.H group at 14 dpe.

Techniques: In Situ Hybridization, RNAscope, Staining, Injection